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Index > Protein center > NOS2(Gene name) > Human
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  • NOS2 (Gene name),
  • Nitric oxide synthase, inducible (Protein name ),  NOS2_HUMAN from NCBI database.
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  • Gene name:
    NOS2(NOS2A);
    Protein name:
    Nitric oxide synthase, inducible;
    Alternative:
    Inducible NO synthase(Inducible NOS;iNOS);Hepatocyte NOS(HEP-NOS);Peptidyl-cysteine S-nitrosylase NOS2;NOS type II;
    Organism:
    Human (Homo sapiens). 
    General Annotation
    Sub Unit:
    Homodimer. Binds SLC9A3R1.
    Function:
    Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2.
    Subcellular Location:
    N/A
    Protein Attributes:
    Sequence length:
    1153
    Sequence:
    50:
    MACPWKFLFK | TKFHQYAMNG | EKDINNNVEK | APCATSSPVT | QDDLQYHNLS | 
    100:
    KQQNESPQPL | VETGKKSPES | LVKLDATPLS | SPRHVRIKNW | GSGMTFQDTL | 
    150:
    HHKAKGILTC | RSKSCLGSIM | TPKSLTRGPR | DKPTPPDELL | PQAIEFVNQY | 
    200:
    YGSFKEAKIE | EHLARVEAVT | KEIETTGTYQ | LTGDELIFAT | KQAWRNAPRC | 
    250:
    IGRIQWSNLQ | VFDARSCSTA | REMFEHICRH | VRYSTNNGNI | RSAITVFPQR | 
    300:
    SDGKHDFRVW | NAQLIRYAGY | QMPDGSIRGD | PANVEFTQLC | IDLGWKPKYG | 
    350:
    RFDVVPLVLQ | ANGRDPELFE | IPPDLVLEVA | MEHPKYEWFR | ELELKWYALP | 
    400:
    AVANMLLEVG | GLEFPGCPFN | GWYMGTEIGV | RDFCDVQRYN | ILEEVGRRMG | 
    450:
    LETHKLASLW | KDQAVVEINI | AVLHSFQKQN | VTIMDHHSAA | ESFMKYMQNE | 
    500:
    YRSRGGCPAD | WIWLVPPMSG | SITPVFHQEM | LNYVLSPFYY | YQVEAWKTHV | 
    550:
    WQDEKRRPKR | REIPLKVLVK | AVLFACMLMR | KTMASRVRVT | ILFATETGKS | 
    600:
    EALAWDLGAL | FSCAFNPKVV | CMDKYRLSCL | EEERLLLVVT | STFGNGDCPG | 
    650:
    NGEKLKKSLF | MLKELNNKFR | YAVFGLGSSM | YPRFCAFAHD | IDQKLSHLGA | 
    700:
    SQLTPMGEGD | ELSGQEDAFR | SWAVQTFKAA | CETFDVRGKQ | HIQIPKLYTS | 
    750:
    NVTWDPHHYR | LVQDSQPLDL | SKALSSMHAK | NVFTMRLKSR | QNLQSPTSSR | 
    800:
    ATILVELSCE | DGQGLNYLPG | EHLGVCPGNQ | PALVQGILER | VVDGPTPHQT | 
    850:
    VRLEALDESG | SYWVSDKRLP | PCSLSQALTY | FLDITTPPTQ | LLLQKLAQVA | 
    900:
    TEEPERQRLE | ALCQPSEYSK | WKFTNSPTFL | EVLEEFPSLR | VSAGFLLSQL | 
    950:
    PILKPRFYSI | SSSRDHTPTE | IHLTVAVVTY | HTRDGQGPLH | HGVCSTWLNS | 
    1000:
    LKPQDPVPCF | VRNASGFHLP | EDPSHPCILI | GPGTGIAPFR | SFWQQRLHDS | 
    1050:
    QHKGVRGGRM | TLVFGCRRPD | EDHIYQEEML | EMAQKGVLHA | VHTAYSRLPG | 
    1100:
    KPKVYVQDIL | RQQLASEVLR | VLHKEPGHLY | VCGDVRMARD | VAHTLKQLVA | 
    1150:
    AKLKLNEEQV | EDYFFQLKSQ | KRYHEDIFGA | VFPYEAKKDR | VAVQPSSLEM | 
    1153:
    SAL
    3D Structure:
    N/A
    Predicted Eptitope:
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    EIAab Sequence  Vaild Sequence:
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    Related Databases
    UniGene:
    SMR:
    Pfam:
    MIM:
    String:
    KEGG:
    Uniprot:
     
    FOR
    ELISA Kit for Human Nitric oxide synthase, inducible
    MSDS:
    Please sign in first.
    ELISA Kit for Human Nitric oxide synthase, inducible
    Cat.:
    E0837r
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Human Nitric oxide synthase, inducible
    Cat.:
    E0837m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Human Nitric oxide synthase, inducible
    Cat.:
    E0837h
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Human Nitric oxide synthase, inducible
    Cat.:
    E0837b
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Human Nitric oxide synthase, inducible
    Cat.:
    E0837p
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Human Nitric oxide synthase, inducible
    Cat.:
    E0837d
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    ELISA Kit for Human Nitric oxide synthase, inducible
    Cat.:
    E0837c
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Human Nitric oxide synthase, inducible
    Cat.:
    U0837m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Human Nitric oxide synthase, inducible
    Cat.:
    U0837d
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Human Nitric oxide synthase, inducible
    MSDS:
    Please sign in first.
    CLIA Kit for Human Nitric oxide synthase, inducible
    MSDS:
    Please sign in first.
    CLIA Kit for Human Nitric oxide synthase, inducible
    Cat.:
    U0837b
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Human Nitric oxide synthase, inducible
    Cat.:
    U0837c
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Human Nitric oxide synthase, inducible
    Cat.:
    U0837r
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Human Nitric oxide synthase, inducible
    Cat.:
    U0837h
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Polyclonal Antibody for Human Nitric oxide synthase, inducible
    Cat.:
    P0837Rb-h
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Human Nitric oxide synthase, inducible
    Polyclonal Antibody for Human Nitric oxide synthase, inducible
    Polyclonal Antibody for Human Nitric oxide synthase, inducible
    Cat.:
    P0837Rb-m
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Human Nitric oxide synthase, inducible
    Polyclonal Antibody for Human Nitric oxide synthase, inducible
    Polyclonal Antibody for Human Nitric oxide synthase, inducible
    Polyclonal Antibody for Human Nitric oxide synthase, inducible
    Cat.:
    P0837Rb-r
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Monoclonal Antibody for Human Nitric oxide synthase, inducible
    Monoclonal Antibody for Human Nitric oxide synthase, inducible
    Monoclonal Antibody for Human Nitric oxide synthase, inducible
    Monoclonal Antibody for Human Nitric oxide synthase, inducible
    Monoclonal Antibody for Human Nitric oxide synthase, inducible
    Monoclonal Antibody for Human Nitric oxide synthase, inducible
    Monoclonal Antibody for Human Nitric oxide synthase, inducible
    Monoclonal Antibody for Human Nitric oxide synthase, inducible
    Protein for Human Nitric oxide synthase, inducible
    Protein for Human Nitric oxide synthase, inducible
    Cat.:
    R0837h
    Price:
    Please sign in first.
    Packing:
    1mg
    Purity:
    >90%
    Protein for Human Nitric oxide synthase, inducible
    Protein for Human Nitric oxide synthase, inducible
    Protein for Human Nitric oxide synthase, inducible
    Protein for Human Nitric oxide synthase, inducible
    Protein for Human Nitric oxide synthase, inducible
    Protein for Human Nitric oxide synthase, inducible

    R&D Technical Data
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    s

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     12% SDS-PAGE analysis

                 12% SDS-PAGE analysis

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    s
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    Precision
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    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤3.9%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤7.7%

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    Recovery
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    Recovery was determined by spiking various levels of ACE into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    99

    91-103

    Plasma

    98

    93-106

     

     

     

     

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    Linearity
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    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    92-105%

    90-101%

    95-105%

    92-109%

    Saliva(n=3)

    89-95%

    93105%

    89-94%

    90-106%

    heparin plasma(n=5)

    93-104%

     

    94-110%

    101-111%

    91-110%

     

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    Citations
    [1].
    Yun-Feng Ni, Jian-Ke Kuai, Zi-Fan Lu, Guo-Dong Yang, Hai-Yan Fu, Jian Wang, Feng Tian, Xiao-Long Yan, Ya-Chao Zhao, Yun-Jie Wang, and Tao Jiang,
    References
    1. 1.
      "Purification and cDNA sequence of an inducible nitric oxide synthase from a human tumor cell line."
      Sherman P.A. , Laubach V.E. , Reep B.R. , Wood E.R.
      Biochemistry32:11600-11605(1993) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1)
      tissue: Colon adenocarcinoma.
    2. 2.
      "Molecular cloning and expression of inducible nitric oxide synthase from human hepatocytes."
      Geller D.A. , Lowenstein C.J. , Shapiro R.A. , Nussler A.K. , di Silvio M. , Wang S.C. , Nakayama D.K. , Simmons R.L. , Snyder S.H. , Billiar T.R.
      Proc. Natl. Acad. Sci. U.S.A.90:3491-3495(1993) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1)
      tissue: Liver.
    3. 3.
      "Cloning, characterization, and expression of a cDNA encoding an inducible nitric oxide synthase from the human chondrocyte."
      Charles I.G. , Palmer R.M.J. , Hickery M.S. , Bayliss M.T. , Chubb A.P. , Hall V.S. , Moss D.W. , Moncada S.
      Proc. Natl. Acad. Sci. U.S.A.90:11419-11423(1993) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1)
      tissue: Chondrocyte.
    4. 4.
      "Inducible nitric oxide synthase from human articular chondrocytes: cDNA cloning and analysis of mRNA expression."
      Maier R. , Bilbe G. , Rediske J. , Lotz M.
      Biochim. Biophys. Acta1208:145-150(1994) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1)
      tissue: Articular chondrocyte.
    5. 5.
      "Human retina expresses both constitutive and inducible isoforms of nitric oxide synthase mRNA."
      Park C.S. , Pardhasaradhi K. , Gianotti C. , Villegas E. , Krishna G.
      Biochem. Biophys. Res. Commun.205:85-91(1994) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1);VARIANT LEU-608
      tissue: Retina.
    6. 6.
      "Cloning and functional expression of human inducible nitric oxide synthase (NOS) cDNA from a glioblastoma cell line A-172."
      Hokari A. , Zeniya M. , Esumi H.
      J. Biochem.116:575-581(1994) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1);VARIANT LEU-608
      tissue: Glioblastoma.
    7. 7.
      "Continuous nitric oxide synthesis by inducible nitric oxide synthase in normal human airway epithelium in vivo."
      Guo F.H. , de Raeve R.H. , Rice T.W. , Stuehr D.J. , Thunnissen F.B.J.M. , Erzurum S.C.
      Proc. Natl. Acad. Sci. U.S.A.92:7809-7813(1995) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1)
      tissue: Airway epithelium.
    8. 8.
      "Dedifferentiated human ventricular cardiac myocytes express inducible nitric oxide synthase mRNA but not protein in response to IL-1, TNF, IFNgamma, and LPS."
      Luss H. , Li R.-K. , Shapiro R.A. , Tzeng E. , McGowan F.X. , Yoneyama T. , Hatakayama K. , Geller D.A. , Mickle D.A.G. , Simmons R.L. , Billiar T.R.
      J. Mol. Cell. Cardiol.29:1153-1165(1997) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1)
      tissue: Cardiac myocyte.
    9. 9.
      "Cloning and characterization of a novel splice valiant of human inducible nitric oxide synthase."
      Ogawa Y. , Nishijima S. , Goto M. , Ida M.
      Submitted (1999-01) to the EMBL/GenBank/DDBJ databases
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2)
    10. 10.
      NIEHS SNPs program
      Submitted (2005-05) to the EMBL/GenBank/DDBJ databases
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA];VARIANTS TRP-221; LEU-608; ALA-747 AND CYS-1009
    11. 11.
      NHLBI resequencing and genotyping service (RS&G)
      Submitted (2007-12) to the EMBL/GenBank/DDBJ databases
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
    12. 12.
      "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1)
    13. 13.
      "Nitric oxide production by human proximal tubular cells: a novel immunomodulatory mechanism?"
      McLay J.S. , Chatterjee P. , Nicolson A.G. , Jardine A.G. , McKay N.G. , Ralston S.H. , Grabowski P. , Haites N.E. , Macleod A.M. , Hawksworth G.M.
      Kidney Int.46:1043-1049(1994) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 380-473
      tissue: Kidney.
    14. 14.
      "Inducible nitric oxide synthase in a human glioblastoma cell line."
      Fujisawa H. , Ogura T. , Hokari A. , Weisz A. , Yamashita J. , Esumi H.
      J. Neurochem.64:85-91(1995) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 667-831;INDUCTION
      tissue: Glioblastoma.
    15. 15.
      "Three members of the nitric oxide synthase II gene family (NOS2A, NOS2B, and NOS2C) colocalize to human chromosome 17."
      Bloch K.D. , Wolfram J.R. , Brown D.M. , Roberts J.D. Jr. , Zapol D.G. , Lepore J.J. , Filippov G. , Thomas J.E. , Jacob H.J. , Bloch D.B.
      Genomics27:526-530(1995) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: CHARACTERIZATION
    16. 16.
      "Inducible nitric oxide synthase in the liver: regulation and function."
      Taylor B.S. , Alarcon L.H. , Billiar T.R.
      Biochemistry (Mosc.)63:766-781(1998) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: CHARACTERIZATION
    17. 17.
      "Epithelial inducible nitric-oxide synthase is an apical EBP50-binding protein that directs vectorial nitric oxide output."
      Glynne P.A. , Darling K.E.A. , Picot J. , Evans T.J.
      J. Biol. Chem.277:33132-33138(2002) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH SLC9A3R1
    18. 18.
      "A new NOS2 promoter polymorphism associated with increased nitric oxide production and protection from severe malaria in Tanzanian and Kenyan children."
      Hobbs M.R. , Udhayakumar V. , Levesque M.C. , Booth J. , Roberts J.M. , Tkachuk A.N. , Pole A. , Coon H. , Kariuki S. , Nahlen B.L. , Mwaikambo E.D. , Lal A.L. , Granger D.L. , Anstey N.M. , Weinberg J.B.
      Lancet360:1468-1475(2002) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: POLYMORPHISM;INVOLVEMENT IN RESISTANCE TO MALARIA
    19. 19.
      "Crystal structures of zinc-free and -bound heme domain of human inducible nitric-oxide synthase. Implications for dimer stability and comparison with endothelial nitric-oxide synthase."
      Li H. , Raman C.S. , Glaser C.B. , Blasko E. , Young T.A. , Parkinson J.F. , Whitlow M. , Poulos T.L.
      J. Biol. Chem.274:21276-21284(1999) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.55 ANGSTROMS) OF 74-504
    20. 20.
      "Structural characterization of nitric oxide synthase isoforms reveals striking active-site conservation."
      Fischmann T.O. , Hruza A. , Niu X.D. , Fossetta J.D. , Lunn C.A. , Dolphin E. , Prongay A.J. , Reichert P. , Lundell D.J. , Narula S.K. , Weber P.C.
      Nat. Struct. Biol.6:233-242(1999) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.25 ANGSTROMS) OF 82-528
    21. 21.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANT [LARGE SCALE ANALYSIS] SER-679
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